nbp2 25221 Search Results


anti muc2 antibody  (Novus Biologicals)
93
Novus Biologicals anti muc2 antibody
Fig. 6. Characterization of the novel human duodenal organoid-derived monolayer model. (a) Immunofluorescence analysis for E-cadherin (red), ZO-1 (green), (b) <t>MUC2</t> (red), and nucleus (blue). Scale bars, 100 µm. (c) Immunofluorescence analysis depicting cross sections of the monolayer model; ZO-1 (green), nucleus (blue), and F-actin (white). Scale bars, 100 µm. Black arrows indicate the Transwell membrane. (d) and (e) Transmission electron microscopic analysis of the monolayer model. Mv, microvilli; Tj, tight junction; Sg, secretory granule-like structures. Scale bars, 10 µm for low magnification images and 2 µm for high magnification images. (f) Efflux transporter P-gp assay using the P-gp substrate digoxin with or without zosuquidar as an inhibitor. AtoB, apical to basal; BtoA, basal to apical. (g) Efflux transporter assay using BCRP substrate sulfasalazine with or without Ko143. (h) UDP-glucuronosyltransferase (UGT) functional assay using raloxifene as a UGT substrate. (i) Carboxylesterase 2 (CES2) functional assay using irinotecan as a substrate with or without loperamide as an inhibitor. The amount of SN-38, a metabolite of irinotecan, was detected in the cells of the monolayer model. (j) and (k) Midazolam permeability and metabolism assay using 2 µM midazolam as a substrate of CYP3A with or without 1-aminobenzotriazole (ABT) as a pan-CYP inhibitor. BLQ, below limit of quantification. (l) Relative gene expression determined by quantitative RT-PCR of the monolayer model with induction of VD3 or VD3 + rifampicin. Cells were treated with inducers for the last two days of the differentiation process. The P value was determined by a two-tailed unpaired t-test.
Anti Muc2 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nbp2+25221/MUC2+Antibody+(CCP58)/pm40181053-332-23-26
Average 93 stars, based on 1 article reviews
anti muc2 antibody - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
nbp2-25221  (novus biologicals)
91
novus biologicals nbp2-25221
Table of Antibodies Used in Study
Nbp2 25221, supplied by novus biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nbp2+25221/MUC2+Antibody+(CCP58)/pmc06009013-7-0-10
Average 91 stars, based on 1 article reviews
nbp2-25221 - by Bioz Stars, 2026-09
91/100 stars
  Buy from Supplier

Image Search Results


Fig. 6. Characterization of the novel human duodenal organoid-derived monolayer model. (a) Immunofluorescence analysis for E-cadherin (red), ZO-1 (green), (b) MUC2 (red), and nucleus (blue). Scale bars, 100 µm. (c) Immunofluorescence analysis depicting cross sections of the monolayer model; ZO-1 (green), nucleus (blue), and F-actin (white). Scale bars, 100 µm. Black arrows indicate the Transwell membrane. (d) and (e) Transmission electron microscopic analysis of the monolayer model. Mv, microvilli; Tj, tight junction; Sg, secretory granule-like structures. Scale bars, 10 µm for low magnification images and 2 µm for high magnification images. (f) Efflux transporter P-gp assay using the P-gp substrate digoxin with or without zosuquidar as an inhibitor. AtoB, apical to basal; BtoA, basal to apical. (g) Efflux transporter assay using BCRP substrate sulfasalazine with or without Ko143. (h) UDP-glucuronosyltransferase (UGT) functional assay using raloxifene as a UGT substrate. (i) Carboxylesterase 2 (CES2) functional assay using irinotecan as a substrate with or without loperamide as an inhibitor. The amount of SN-38, a metabolite of irinotecan, was detected in the cells of the monolayer model. (j) and (k) Midazolam permeability and metabolism assay using 2 µM midazolam as a substrate of CYP3A with or without 1-aminobenzotriazole (ABT) as a pan-CYP inhibitor. BLQ, below limit of quantification. (l) Relative gene expression determined by quantitative RT-PCR of the monolayer model with induction of VD3 or VD3 + rifampicin. Cells were treated with inducers for the last two days of the differentiation process. The P value was determined by a two-tailed unpaired t-test.

Journal: Scientific reports

Article Title: Robust and reproducible human intestinal organoid-derived monolayer model for analyzing drug absorption.

doi: 10.1038/s41598-025-95823-z

Figure Lengend Snippet: Fig. 6. Characterization of the novel human duodenal organoid-derived monolayer model. (a) Immunofluorescence analysis for E-cadherin (red), ZO-1 (green), (b) MUC2 (red), and nucleus (blue). Scale bars, 100 µm. (c) Immunofluorescence analysis depicting cross sections of the monolayer model; ZO-1 (green), nucleus (blue), and F-actin (white). Scale bars, 100 µm. Black arrows indicate the Transwell membrane. (d) and (e) Transmission electron microscopic analysis of the monolayer model. Mv, microvilli; Tj, tight junction; Sg, secretory granule-like structures. Scale bars, 10 µm for low magnification images and 2 µm for high magnification images. (f) Efflux transporter P-gp assay using the P-gp substrate digoxin with or without zosuquidar as an inhibitor. AtoB, apical to basal; BtoA, basal to apical. (g) Efflux transporter assay using BCRP substrate sulfasalazine with or without Ko143. (h) UDP-glucuronosyltransferase (UGT) functional assay using raloxifene as a UGT substrate. (i) Carboxylesterase 2 (CES2) functional assay using irinotecan as a substrate with or without loperamide as an inhibitor. The amount of SN-38, a metabolite of irinotecan, was detected in the cells of the monolayer model. (j) and (k) Midazolam permeability and metabolism assay using 2 µM midazolam as a substrate of CYP3A with or without 1-aminobenzotriazole (ABT) as a pan-CYP inhibitor. BLQ, below limit of quantification. (l) Relative gene expression determined by quantitative RT-PCR of the monolayer model with induction of VD3 or VD3 + rifampicin. Cells were treated with inducers for the last two days of the differentiation process. The P value was determined by a two-tailed unpaired t-test.

Article Snippet: The monolayer was incubated with primary antibodies [anti-ZO-1 antibody Alexa Fluor 488 (MA3-39,100-A488; Thermo Fisher Scientific), anti-E-cadherin antibody (PA5-32,178; Thermo Fisher Scientific), and anti-MUC2 antibody (NBP225,221; Novus Biologicals)] in blocking solution containing 0.3% Triton X-100 at 4 °C overnight.

Techniques: Derivative Assay, Immunofluorescence, Membrane, Transmission Assay, Functional Assay, Permeability, Gene Expression, Quantitative RT-PCR, Two Tailed Test

Table of Antibodies Used in Study

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Enhanced Utilization of Induced Pluripotent Stem Cell–Derived Human Intestinal Organoids Using Microengineered Chips

doi: 10.1016/j.jcmgh.2017.12.008

Figure Lengend Snippet: Table of Antibodies Used in Study

Article Snippet: Mucin-2 (CCP58) , 1:500 , NBP2-25221 , Mouse IgG1 , Novus Biologicals.

Techniques: